The Animal Protection Services of Saskatchewan (APSS) incorporated as a non-profit charity in 2015 to undertake animal protection work and cruelty investigations outside of Regina and Saskatoon.  There are currently seven full-time animal protection officers employed by APSS. In 2015 the Ministry of Agriculture began funding the investigative services with $610,000. Funding is still provided by the Ministry of Agriculture until March 31, 2021. The central location is based out of Saskatoon, with another office located in Moose Jaw.

The enactment of the Animal Protection Act, 2018 gives policing powers to members of the RCMP and a municipal police officer to enforce the provincial Act around the province.

Regina SPCA is an animal charity responsible for investigation animal welfare concerns and suspected animal abuse in the City of Regina. There are three full-time APOs and one full-time Dispatch Coordinator responsible for investigations in the city. Officers investigate an average of 600 complaints annually. Approximately $300,000 is spent to conduct the investigations, care for the animals, and prepare charges. The Regina SPCA does not receive any provincial funding to conduct this work and relies on donations, grants, and self-generated income.

The organization has two Animal Protection Officers who respond to over 900 reports annually. The Saskatoon SPCA does not receive government funding and relies on donations, grants, and self-generated income to operate.  In 1973, the Saskatoon SPCA Auxiliary was formed as a separate fundraising section of the SPCA.  In 2018, the city paid an annual grant of $159,540 for Animal Protection and Cruelty Investigation Services. In a 2018 financial report the operating funds of animal care and protection was reported as $371,332, up from $341,708 in 2017.

Saskatchewan Environment, also known as the Ministry of Environment, is responsible for investigating any fishing, wildlife or environmental violations. Conservation officers are responsible for the protection of fish and wildlife.  They have the same legal authority of police officers when enforcing wildlife and environmental laws.  There are 44 field offices, with 19 Sergeants that supervise 94 Conservation Officers.  One inspector manages seven offices. Additionally, there is also a K9 unit, a Training and Education unit and a Special Investigations Unit. The officers are part of the Saskatchewan Association of Conservation Officers.